LAMP for Rapid Detection of Common Pathogens causing disease in Swine

 

LAMP stands for Loop-mediated Isothermal Amplification. It is a simple rapid, specific and cost-effective diagnostic tool that is based on the dna amplification method.

The LAMP method is comparable to PCR  (polymerase chain reaction) in terms of sensitivity and specificity. In contrast to PCR however, the LAMP method is less costly and less affected by the presence of non-target DNA and inhibitory contaminants. LAMP does not require sophisticated equipment and reagents. Also, it can be used with simple heating devise such as water bath or heat block. Because of its high specificity and visiual indicators of the presence of a pathogen (Notomi et al, 2000) the LAMP method is considered a potential tool for field diagnosis of swine diseases. Continue reading “LAMP for Rapid Detection of Common Pathogens causing disease in Swine”